Human Papillomavirus (Mitundu 28) Genotyping Detection Kit (Fluorescence PCR)

Chidacho chimagwiritsa ntchito njira yodziwira ma nucleic acid amplification (PCR) fluorescence.Zoyambira zapadera kwambiri ndi zofufuzira zidapangidwa kutengera mtundu wa L1 wa HPV.Kafukufukuyu amalembedwa ndi FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore, HP435 , 45, 54, 56, 68, 82) ndi ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) pa 5', ndipo gulu la 3' quencher ndi BHQ1 kapena BHQ2.Pakukulitsa kwa PCR, zoyambira zenizeni ndi zofufuzira zimamangiriza kutsatana kwawo.Pamene Taq enzyme ikukumana ndi ma probes omwe amamangiriridwa ku ndondomeko ya chandamale, imagwira ntchito ya 5 'end exonuclease kuti ilekanitse mtolankhani wa fluorophore kuchokera ku quencher fluorophore, kotero kuti njira yowunikira fulorosenti ikhoza kulandira chizindikiro cha fulorosenti, ndiko kuti, nthawi iliyonse DNA. strand imakulitsa, molekyulu ya fulorosenti imapangidwa, yomwe imazindikira kulunzanitsa kwathunthu kwa kudzikundikira kwa ma siginecha a fulorosenti ndi mapangidwe azinthu za PCR, kuti akwaniritse kuzindikira kwamtundu wa nucleic acid wamitundu 28 ya papillomavirus yamunthu mu khomo lachiberekero exfoliated cell zitsanzo. .