Herpes Simplex Virus Type 2 Nucleic Acid

Kufotokozera Kwachidule:

Chidachi chimagwiritsidwa ntchito pozindikira zamtundu wa herpes simplex virus mtundu 2 nucleic acid mu swab wamwamuna wa mkodzo ndi zitsanzo za khomo lachiberekero.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina lazogulitsa

HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Cholinga Chogwiritsidwa Ntchito

Chidachi chimagwiritsidwa ntchito pozindikira zamtundu wa herpes simplex virus mtundu 2 nucleic acid mu swab wamwamuna wa mkodzo ndi zitsanzo za khomo lachiberekero.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) ndi kachilombo kozungulira kopangidwa ndi tegument, capsid, core, ndi envelopu, ndipo imakhala ndi mizere iwiri ya DNA.Matenda a Herpes amatha kulowa m'thupi mwa kukhudzana mwachindunji kapena kugonana ndi khungu ndi mucous nembanemba, ndipo amagawidwa kukhala oyambirira komanso obwerezabwereza.Matenda a ubereki amayamba makamaka ndi HSV2, odwala amuna amawonetsedwa ngati zilonda za mbolo, ndipo odwala achikazi amawonetsedwa ngati zilonda zam'mimba, vulvar, ndi maliseche.The koyamba matenda a maliseche nsungu HIV nthawi zambiri recessive matenda, kupatula ochepa m`deralo nsungu ndi mucous nembanemba kapena khungu, ambiri amene alibe zizindikiro zachipatala zoonekeratu.Matenda a genital nsungu ali ndi makhalidwe a moyo wonse wonyamula kachilombo ka HIV ndi kubwereza kosavuta, ndipo odwala ndi onyamula ndi omwe amayambitsa matenda.Ku China, kuchuluka kwa serological positive kwa HSV2 kuli pafupifupi 10.80% mpaka 23.56%.Gawo la matenda a HSV2 likhoza kugawidwa kukhala matenda oyamba ndi matenda obwerezabwereza, ndipo pafupifupi 60% ya odwala omwe ali ndi kachilombo ka HSV2 amayambiranso.

Epidemiology

FAM: Herpes Simplex Virus Type 2 (HSV2)·

VIC(HEX): Kulamulira Kwamkati

 

PCR Amplification Conditions Kukhazikitsa

Khwerero

Zozungulira

Kutentha

Nthawi

SunganiFluorescentSmagalasikapena osati

1

1 kuzungulira

50 ℃

5 mins

No

2

1 kuzungulira

95 ℃

10 mins

No

3

40 kuzungulira

95 ℃

15mphindi

No

4

58 ℃

31mphindi

Inde

Magawo aukadaulo

Kusungirako  
Madzi

≤-18 ℃ Mumdima

Alumali moyo 12 miyezi
Mtundu wa Chitsanzo

Mphepete mwa khomo lachiberekero lachikazi, Male swab

Ct

≤38

CV

≤5.0%

LoD 50Makope/machitidwe
Mwatsatanetsatane

Palibe mtanda-reactivity ndi matenda ena opatsirana pogonana, monga Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium ndi etc.

Zida Zogwiritsira Ntchito

Itha kufanana ndi zida za fulorosenti za PCR pamsika.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System.

Kuyenda Ntchito

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