Staphylococcus Aureus ndi Methicillin-Resistant Staphylococcus Aureus
Dzina la malonda
HWTS-OT062 Staphylococcus Aureus ndi Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit(Fluorescence PCR)
Satifiketi
CE
Epidemiology
Staphylococcus aureus ndi amodzi mwa mabakiteriya ofunikira amtundu wa nosocomial.Staphylococcus aureus (SA) ndi ya staphylococcus ndipo imayimira mabakiteriya a Gram-positive, omwe amatha kupanga mitundu yambiri ya poizoni ndi ma enzymes owononga.Mabakiteriya ali ndi mawonekedwe a kugawa kwakukulu, mphamvu yamphamvu ya pathogenicity komanso kukana kwakukulu.Thermostable nuclease jini (nuc) ndi jini yotetezedwa kwambiri ya staphylococcus aureus.
Channel
| FAM | jini ya mecA yolimbana ndi methicillin |
| Mtengo ROX | Ulamuliro Wamkati |
| CY5 | staphylococcus aureus nuc gene |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ & kutetezedwa ku kuwala |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | sputum, zitsanzo za matenda a pakhungu ndi minofu yofewa, ndi zitsanzo za swab za m'mphuno |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL mabakiteriya osamva methicillin.Chidacho chikazindikira mtundu wa LoD, 1000/mL staphylococcus aureus imatha kudziwika. |
| Mwatsatanetsatane | Mayeso a cross-reactivity akuwonetsa kuti zidazi zilibe mgwirizano ndi tizilombo toyambitsa matenda ena opuma monga methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichinicbacoccus, coagulase-negative staphylococcus. ife mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. itha kugwiritsidwa ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Onjezani 200µL ya saline wamba pamadzi okonzedwa, ndipo masitepe otsatirawa atengedwe molingana ndi malangizo, ndipo voliyumu yoyeserera ndi 80µL.
Njira 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 1mL ya saline wamba pamadzi mutatha kutsuka ndi saline wamba, kenaka sakanizani bwino.Centrifuge pa 13,000r/mphindi kwa mphindi 5, chotsani supernatant (sungani 10-20µL ya supernatant), ndipo tsatirani malangizo a m'zigawo zotsatira.
Regent yovomerezeka yochotsa: Nucleic Acid Extraction kapena Purification Reagent (YDP302) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd.Kuchotsa kuyenera kuchitidwa mosamalitsa molingana ndi gawo 2 la buku la malangizo.Ndibwino kuti mugwiritse ntchito madzi a RNase ndi DNase kuti mutengeko ndi mphamvu ya 100µL.
