Munthu ROS1 Fusion Gene Mutation

Kufotokozera Kwachidule:

Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative qualitative mitundu 14 ya masinthidwe amtundu wa ROS1 fusion mu zitsanzo za khansa ya m'mapapo yamunthu yomwe si yaying'ono (Table 1).Zotsatira zoyezetsa ndizongofotokozera zachipatala zokha ndipo siziyenera kugwiritsidwa ntchito ngati njira yokhayo yothandizira odwala payekhapayekha.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Satifiketi

CE

Epidemiology

ROS1 ndi transmembrane tyrosine kinase ya banja la insulin receptor.ROS1 fusion jini yatsimikiziridwa ngati jini ina yofunika yoyendetsa khansa ya m'mapapo yopanda maselo yaying'ono.Monga nthumwi ya mtundu watsopano wapadera wa ma molekyulu, zochitika za ROS1 fusion jini mu NSCLC Pafupifupi 1% mpaka 2% ROS1 makamaka imakumana ndi kusintha kwa majini mu ma exons 32, 34, 35 ndi 36. Ikaphatikizidwa ndi majini monga CD74, EZR, SLC34A2, ndi SDC4, ipitiliza kuyambitsa dera la ROS1 tyrosine kinase.ROS1 kinase yolumikizidwa mosadziwika bwino imatha kuyambitsa njira zolozera kumunsi monga RAS/MAPK/ERK, PI3K/Akt/mTOR, ndi JAK3/STAT3, potero kutenga nawo gawo pakuchulukira, kusiyanitsa ndi kusakhazikika kwa ma cell chotupa, ndikuyambitsa khansa.Pakati pa masinthidwe osakanikirana a ROS1, CD74-ROS1 imakhala pafupifupi 42%, EZR imakhala pafupifupi 15%, SLC34A2 imakhala pafupifupi 12%, ndipo SDC4 imakhala pafupifupi 7%.Kafukufuku wasonyeza kuti ATP-binding site of catalytic domain of ROS1 kinase ndi ATP-binding site of ALK kinase ali ndi homology mpaka 77%, kotero ALK tyrosine kinase yaing'ono molecule inhibitor crizotinib ndi zina zotero zimakhala zoonekeratu machiritso zotsatira. pochiza NSCLC ndi masinthidwe ophatikizika a ROS1.Choncho, kuzindikira kwa kusintha kwa ROS1 fusion ndiye maziko ndi maziko otsogolera kugwiritsa ntchito mankhwala a crizotinib.

Channel

FAM Rection Buffer 1, 2, 3 ndi 4
VIC (HEX) Reaction Buffer 4

Magawo aukadaulo

Kusungirako

≤-18 ℃

Alumali moyo

9 miyezi

Mtundu wa Chitsanzo

parafini-ophatikizidwa minofu pathological kapena sliced ​​zitsanzo

CV

<5.0%

Ct

≤38

LoD

Chida ichi chimatha kuzindikira masinthidwe ophatikizika otsika mpaka makope 20.

Zida Zogwiritsira Ntchito:

Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR Systems

SLAN ®-96P Real-Time PCR Systems

QuantStudio™ 5 Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Kuyenda Ntchito

Zopangira zopangira zovomerezeka: RNeasy FFPE Kit (73504) kuchokera ku QIAGEN, Gawo la Paraffin Embedded Tissue Total RNA Extraction Kit(DP439) kuchokera ku Tiangen Biotech(Beijing) Co.,Ltd.


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