Munthu BRAF Gene V600E Kusintha

Kufotokozera Kwachidule:

Zida zoyeserazi zimagwiritsidwa ntchito kuti zizindikire kusintha kwa BRAF jini V600E mu zitsanzo zamtundu wa parafini wa melanoma yamunthu, khansa yapakhungu, khansa ya chithokomiro ndi khansa ya m'mapapo mu vitro.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)

Satifiketi

CE

Epidemiology

Mitundu yopitilira 30 ya masinthidwe a BRAF yapezeka, yomwe pafupifupi 90% ili mu exon 15, pomwe kusintha kwa V600E kumawonedwa ngati kofala kwambiri, ndiko kuti, thymine(T) pamalo 1799 mu exon 15 imasinthidwa kukhala adenine (A), zomwe zimapangitsa kuti valine (V) alowe m'malo 600 ndi glutamic acid (E) mu mapuloteni.Kusintha kwa BRAF kumapezeka kawirikawiri m'matenda oopsa monga melanoma, khansa yapakhungu, khansa ya chithokomiro, ndi khansa ya m'mapapo.Kumvetsetsa kusintha kwa jini ya BRAF kwakhala kufunikira kowunika ma EGFR-TKIs ndi mankhwala omwe amayang'aniridwa ndi BRAF gene mutation chithandizo chamankhwala cha odwala omwe angapindule nawo.

Channel

FAM Kusintha kwa V600E, kuwongolera mkati

Magawo aukadaulo

Kusungirako

≤-18 ℃

Alumali moyo

9 miyezi

Mtundu wa Chitsanzo

Zitsanzo za minofu ya pathological yokhala ndi parafini

CV

<5.0%

Ct

≤38

LoD

Gwiritsani ntchito zida kuti muzindikire kuwongolera koyenera kwa LoD.a) pansi pa 3ng/μL zakutchire zakutchire, 1% mutation mlingo akhoza kuzindikirika mu anachita nkhokwe stably;b) pansi pa 1% mutation rate, kusintha kwa 1 × 103Makope/mL mumtundu wakutchire wa 1 × 105Makope/mL amatha kuzindikirika mokhazikika muzosungira zomwe zimachitika;c) IC Reaction Buffer imatha kuzindikira malire otsika kwambiri owongolera khalidwe la SW3 la kayendetsedwe ka mkati mwa kampani.

Zida Zogwiritsira Ntchito:

Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7300 Real-Time PCR

Systems, QuantStudio® 5 Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

BioRad CFX96 Real-Time PCR System

Kuyenda Ntchito

Zopangira zopangira zovomerezeka: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yopangidwa ndi Tiangen Biotech(Beijing) Co., Ltd.


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