Munthu BRAF Gene V600E Kusintha
Dzina la malonda
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)
Satifiketi
CE
Epidemiology
Mitundu yopitilira 30 ya masinthidwe a BRAF yapezeka, yomwe pafupifupi 90% ili mu exon 15, pomwe kusintha kwa V600E kumawonedwa ngati kofala kwambiri, ndiko kuti, thymine(T) pamalo 1799 mu exon 15 imasinthidwa kukhala adenine (A), zomwe zimapangitsa kuti valine (V) alowe m'malo 600 ndi glutamic acid (E) mu mapuloteni.Kusintha kwa BRAF kumapezeka kawirikawiri m'matenda oopsa monga melanoma, khansa yapakhungu, khansa ya chithokomiro, ndi khansa ya m'mapapo.Kumvetsetsa kusintha kwa jini ya BRAF kwakhala kufunikira kowunika ma EGFR-TKIs ndi mankhwala omwe amayang'aniridwa ndi BRAF gene mutation chithandizo chamankhwala cha odwala omwe angapindule nawo.
Channel
| FAM | Kusintha kwa V600E, kuwongolera mkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | Zitsanzo za minofu ya pathological yokhala ndi parafini |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Gwiritsani ntchito zida kuti muzindikire kuwongolera koyenera kwa LoD.a) pansi pa 3ng/μL zakutchire zakutchire, 1% mutation mlingo akhoza kuzindikirika mu anachita nkhokwe stably;b) pansi pa 1% mutation rate, kusintha kwa 1 × 103Makope/mL mumtundu wakutchire wa 1 × 105Makope/mL amatha kuzindikirika mokhazikika muzosungira zomwe zimachitika;c) IC Reaction Buffer imatha kuzindikira malire otsika kwambiri owongolera khalidwe la SW3 la kayendetsedwe ka mkati mwa kampani. |
| Zida Zogwiritsira Ntchito: | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7300 Real-Time PCR Systems, QuantStudio® 5 Real-Time PCR Systems LightCycler®480 Real-Time PCR system BioRad CFX96 Real-Time PCR System |
Kuyenda Ntchito
Zopangira zopangira zovomerezeka: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yopangidwa ndi Tiangen Biotech(Beijing) Co., Ltd.
