SARS-CoV-2 chimfine A fuluwenza B Nucleic Acid Kuphatikiza
Dzina la malonda
HWTS-RT060A-SARS-CoV-2 chimfine A B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a Corona Virus 2019 (COVID-19) amayamba ndi SARS-CoV-2 yomwe ndi ya β Coronavirus yamtundu.COVID-19 ndi matenda opatsirana owopsa, ndipo unyinji wa anthu nthawi zambiri umakhala wotengeka.Pakadali pano, odwala omwe ali ndi kachilombo ka SARS-CoV-2 ndiye gwero lalikulu la matendawa, ndipo odwala asymptomatic amathanso kukhala gwero la matenda.Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1-14, makamaka masiku 3-7.Zisonyezero zazikulu zinali malungo, youma chifuwa ndi kutopa.Odwala ochepa amakhala ndi zizindikiro monga kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba.
Fuluwenza ndi pachimake kupuma matenda chifukwa fuluwenza HIV.Imapatsirana kwambiri ndipo imafalikira makamaka chifukwa cha kutsokomola ndi kuyetsemula.Nthawi zambiri imayamba masika ndi nyengo yozizira.Pali mitundu itatu ya Influenza, Influenza A (IFV A), Influenza B (IFV B) ndi Influenza C (IFV C), onsewa ndi a banja la ortomyxovirus.Fuluwenza A ndi B, omwe ali amodzi-stranded, segmental RNA mavairasi, ndi amene amayambitsa matenda a anthu.Fuluwenza A ndi pachimake kupuma matenda opatsirana, kuphatikizapo H1N1, H3N2 ndi subtypes ena, n'zosavuta kusintha.Kufalikira kwapadziko lonse lapansi, "kusintha" kumatanthauza kusintha kwa chimfine A, zomwe zimapangitsa kuti pakhale kachilombo ka "subtype".Fuluwenza B imagawidwa m'mizere iwiri: Yamagata ndi Victoria.Influenza B imangokhala ndi antigenic drift, ndipo amazemba kuyang'aniridwa ndi kuthetsedwa ndi chitetezo cha mthupi mwa munthu kudzera mukusintha.Koma ma virus a fuluwenza B amasintha pang'onopang'ono kuposa fuluwenza A, yomwe imayambitsanso matenda opumira komanso miliri mwa anthu.
Channel
FAM | SARS-CoV-2 |
Mtengo ROX | IFV B |
CY5 | IFV A |
VIC (HEX) | Majini olamulira amkati |
Magawo aukadaulo
Kusungirako | Zamadzimadzi: ≤-18 ℃ Mumdima |
Lyophilization: ≤30 ℃ mumdima | |
Alumali moyo | Madzi: 9 miyezi |
Lyophilization: miyezi 12 | |
Mtundu wa Chitsanzo | Masamba a Nasopharyngeal, Oropharyngeal swabs |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 300 Makopi / ml |
Mwatsatanetsatane | Zotsatira za mayeso a mtanda zidawonetsa kuti zidazo zinali zogwirizana ndi coronavirus yamunthu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, kupuma kwa syncytial virus A ndi B, parainfluenza virus 1, 2 ndi 3, rhinovirus A, B ndi C, adenovirus 1, 2, 3, 4, 5, 7 ndi 55, metapneumovirus yaumunthu, enterovirus A, B, C ndi D, kachilombo ka cytoplasmic pulmonary virus, kachilombo ka EB, kachilombo ka chikuku Human cytomegalovirus, rotavirus, Norovirus, kachilombo ka mumps, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium pneumoniae, Caspylococcus pneumoniae, Casperococcus pneumoniae, Mycobacterium pneumoniae, Casperatalus sanali cross reaction pakati pa Pneumocystis yersini ndi Cryptococcus neoformans. |
Zida Zogwiritsira Ntchito: | Itha kufanana ndi zida za fulorosenti za PCR pamsika. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).
Njira 2.
Zomwe zimalangizidwa m'zigawo: Nucleic Acid Extraction kapena Purification Reagent(YDP302) yolembedwa ndi Tiangen Biotech(Beijing) Co.,Ltd.