Coxsackie Virus Type A16 Nucleic Acid

Chidachi chimatenga Enzymatic Probe Isothermal Amplification (EPIA), ndikupanga zoyambira zenizeni ndi ma probe okhala ndi RNA (rProbe) kudera lotetezedwa kwambiri la Cox A16, ndikuwonjezera Bst enzyme ndi RNaseH enzyme nthawi yomweyo, momwe kumanzere ndi kumanzere. Malekezero akumanja a maziko a RNA a rProbe amalembedwa ndi magulu a fulorosenti ndi chozimitsa, motsatana.Gwiritsani ntchito ntchito ya DNA polymerase ndi ntchito ya strand displacement ya Bst enzyme kuti mukweze chandamale kuti chiyesedwe kutentha kosasintha, enzyme ya RNaseH imatha kuphwanya maziko a RNA pa chain-probe hybrid chain, kuti gulu la fulorosenti ndi chozimitsira cha rProbe. olekanitsidwa potero fluorescing.Kuphatikiza apo, chidutswa chakumanzere cha rProbe RNA yotsalira chitha kugwiritsidwa ntchito ngati choyambira kuti chiwonjezere kupanga chinthu, chomwe chimawonjezeranso chinthucho.Chizindikiro cha fulorosenti chimasonkhanitsidwa mosalekeza ndi mapangidwe a chinthucho, potero kuzindikira kuzindikira kwa chandamale cha nucleic acid.