Mitundu 15 ya Papillomavirus Yowopsa Yamunthu E6/E7 Gene mRNA
Dzina la malonda
HWTS-CC005A-15 Mitundu Yambiri Yangozi Yapapillomavirus Yamunthu E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiology
Khansara ya pachibelekero ndi imodzi mwa mitundu yofala kwambiri ya khansa ya amayi padziko lonse lapansi, ndipo kupezeka kwake kumagwirizana kwambiri ndi ma virus a human papillomavirus (HPV), koma ndi gawo lochepa chabe la matenda a HPV omwe amatha kukhala khansa.HPV yowopsa kwambiri imayambitsa ma cell a epithelial a khomo lachiberekero ndikupanga ma oncoprotein awiri, E6 ndi E7.Puloteniyi imatha kukhudza mapuloteni osiyanasiyana am'ma cell (monga chotupa suppressor proteins pRB ndi p53), kutalikitsa kuzungulira kwa maselo, kumakhudza kaphatikizidwe ka DNA ndi kukhazikika kwa ma genome, ndikusokoneza mayankho a antiviral ndi antitumor.
Channel
| Channel | Chigawo | Genotype anayesedwa |
| FAM | HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Jini ya β-actin yamunthu | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Mtundu wa INS wamunthu |
Magawo aukadaulo
| Kusungirako | Madzi: ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | khomo lachiberekero exfoliated cell |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi / ml |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. M'zigawozi ayenera kuchitidwa mosamalitsa malinga ndi malangizo ntchito .Voliyumu yoyeserera yovomerezeka ndi 50μL.Ngati chitsanzocho sichinagayidwe kwathunthu, chibwezereni ku gawo 4 kuti mugayidwenso.Ndiyeno yesani malinga ndi malangizo ntchito.
Regent yovomerezeka yotsatsira: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).M'zigawozi ziyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito mosamalitsa (Mu gawo 5, wiritsani kuchuluka kwa njira yogwirira ntchito ya DNaseI, ndiye kuti, tengani 20μL ya RNase-Free DNaseI (1500U) yankho la stock mu chubu chatsopano cha RNase-Free centrifuge, onjezani 60μL ya RDD buffer, ndikusakaniza mofatsa).Voliyumu yovomerezeka ndi 60μL.Ngati chitsanzocho sichinagayidwe kwathunthu, chibwezeretseni ku sitepe 5 kuti mugayidwenso.Ndiyeno yesani malinga ndi malangizo ntchito.
